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Protective effect of aucubin on high glucose-induced hGENCs injury by promoting ATG4B protein phosphorylation and increasing autophagy. (A) Electrophoretic map of cells in each group. 1, 2, 3, 4, and 5 represent Con group, HG group, HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (B) Mass spectrogram of protein identification. Venn diagram of (C) phosphorylated proteins, (D) phosphorylated protein sites and (E) protein identification, AU1, AU2 and AU3 represent HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (F) Representative images of expression levels of p-ATG4B and ATG4B detected by WB. (G) Representative images of the expression levels of autophagy protein LC3, p62, <t>ATG5,</t> and ATG7 in each group detected by WB, and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. AU: aucubin; HG: high glucose.
Atg5, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Protective effect of aucubin on high glucose-induced hGENCs injury by promoting ATG4B protein phosphorylation and increasing autophagy. (A) Electrophoretic map of cells in each group. 1, 2, 3, 4, and 5 represent Con group, HG group, HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (B) Mass spectrogram of protein identification. Venn diagram of (C) phosphorylated proteins, (D) phosphorylated protein sites and (E) protein identification, AU1, AU2 and AU3 represent HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (F) Representative images of expression levels of p-ATG4B and ATG4B detected by WB. (G) Representative images of the expression levels of autophagy protein LC3, p62, <t>ATG5,</t> and ATG7 in each group detected by WB, and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. AU: aucubin; HG: high glucose.
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Average 96 stars, based on 1 article reviews
rabbit anti atg5 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
anti atg5  (Proteintech)
96
Proteintech anti atg5
Protective effect of aucubin on high glucose-induced hGENCs injury by promoting ATG4B protein phosphorylation and increasing autophagy. (A) Electrophoretic map of cells in each group. 1, 2, 3, 4, and 5 represent Con group, HG group, HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (B) Mass spectrogram of protein identification. Venn diagram of (C) phosphorylated proteins, (D) phosphorylated protein sites and (E) protein identification, AU1, AU2 and AU3 represent HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (F) Representative images of expression levels of p-ATG4B and ATG4B detected by WB. (G) Representative images of the expression levels of autophagy protein LC3, p62, <t>ATG5,</t> and ATG7 in each group detected by WB, and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. AU: aucubin; HG: high glucose.
Anti Atg5, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti atg5/product/Proteintech
Average 96 stars, based on 1 article reviews
anti atg5 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier

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Protective effect of aucubin on high glucose-induced hGENCs injury by promoting ATG4B protein phosphorylation and increasing autophagy. (A) Electrophoretic map of cells in each group. 1, 2, 3, 4, and 5 represent Con group, HG group, HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (B) Mass spectrogram of protein identification. Venn diagram of (C) phosphorylated proteins, (D) phosphorylated protein sites and (E) protein identification, AU1, AU2 and AU3 represent HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (F) Representative images of expression levels of p-ATG4B and ATG4B detected by WB. (G) Representative images of the expression levels of autophagy protein LC3, p62, ATG5, and ATG7 in each group detected by WB, and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. AU: aucubin; HG: high glucose.

Journal: Renal Failure

Article Title: Aucubin ameliorates diabetic kidney disease by restoring hGENCs autophagy through promoting phosphorylation of ATG4B protein

doi: 10.1080/0886022X.2025.2605756

Figure Lengend Snippet: Protective effect of aucubin on high glucose-induced hGENCs injury by promoting ATG4B protein phosphorylation and increasing autophagy. (A) Electrophoretic map of cells in each group. 1, 2, 3, 4, and 5 represent Con group, HG group, HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (B) Mass spectrogram of protein identification. Venn diagram of (C) phosphorylated proteins, (D) phosphorylated protein sites and (E) protein identification, AU1, AU2 and AU3 represent HG-AU-L group, HG-AU-M group, and HG-AU-H group, respectively. (F) Representative images of expression levels of p-ATG4B and ATG4B detected by WB. (G) Representative images of the expression levels of autophagy protein LC3, p62, ATG5, and ATG7 in each group detected by WB, and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. AU: aucubin; HG: high glucose.

Article Snippet: Proteins were transferred to polyvinylidene difluoride membranes (PVDF) and then incubated with specific primary antibodies against ATG4B, p-ATG4B(Ser383), ATG5, ATG7 (1:1,000, CST); Bcl-2, Bax (1:500, Immunoway); p62, cleaved caspase3 (1:500, Immunoway); LC3 (1:1,000, Abcam); CD31, Vimentin (1:2,000, Proteintech); nephrin (1:1,000, Affinity) and α-SMA, GAPDH(1:1,000, Proteintech), and secondary antibodies conjugated to HRP (Proteintech).

Techniques: Phospho-proteomics, Expressing

Protective effect of aucubin on DKD mice by promoting ATG4B protein phosphorylation and increasing autophagy. The expression levels of p-ATG4B proteins, ATG4B proteins, autophagy protein (LC3, p62, ATG5, and ATG7), apoptosis-related proteins (Bax, cleaved caspase3, and Bcl-2), EndMT marker proteins (CD31 and α-SMA), and nephrin protein in each group were detected by WB and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. DKD: diabetic kidney disease; AU: aucubin.

Journal: Renal Failure

Article Title: Aucubin ameliorates diabetic kidney disease by restoring hGENCs autophagy through promoting phosphorylation of ATG4B protein

doi: 10.1080/0886022X.2025.2605756

Figure Lengend Snippet: Protective effect of aucubin on DKD mice by promoting ATG4B protein phosphorylation and increasing autophagy. The expression levels of p-ATG4B proteins, ATG4B proteins, autophagy protein (LC3, p62, ATG5, and ATG7), apoptosis-related proteins (Bax, cleaved caspase3, and Bcl-2), EndMT marker proteins (CD31 and α-SMA), and nephrin protein in each group were detected by WB and the statistical analysis result of the proteins expression levels. *p < .05; ** p < .01. DKD: diabetic kidney disease; AU: aucubin.

Article Snippet: Proteins were transferred to polyvinylidene difluoride membranes (PVDF) and then incubated with specific primary antibodies against ATG4B, p-ATG4B(Ser383), ATG5, ATG7 (1:1,000, CST); Bcl-2, Bax (1:500, Immunoway); p62, cleaved caspase3 (1:500, Immunoway); LC3 (1:1,000, Abcam); CD31, Vimentin (1:2,000, Proteintech); nephrin (1:1,000, Affinity) and α-SMA, GAPDH(1:1,000, Proteintech), and secondary antibodies conjugated to HRP (Proteintech).

Techniques: Phospho-proteomics, Expressing, Marker